Description
Human BUB1 Recombinant Protein (N-His)
Human BUB1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-His151, with an N-terminal His tag. Predicted molecular weight: 20.25 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Mitotic checkpoint serine/threonine-protein kinase BUB1 is a ~122 kDa protein. Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. For research use only.
Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion.
| Product Name: | Human BUB1 Recombinant Protein (N-His) |
| SKU: | GXRP05666 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | BUB1L, hBUB1, BUB1A, Mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-His151 |
| Calculated MW: | 20.25 kDa |
| UniProt: | O43683 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
