The Human C4 Complement Component 4 CLIA Kit is specifically designed for the precise measurement of C4 complement component 4 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.The C4 complement component 4 is a vital protein involved in the complement system, playing a crucial role in immune responses and inflammation.
Dysregulation of C4 levels has been associated with autoimmune diseases, such as lupus and rheumatoid arthritis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human C4 Complement Component 4 CLIA Kit provides researchers with a valuable tool for conducting in-depth investigations into the role of C4 in health and disease, ultimately contributing to the development of novel treatment strategies.
Product Name:
Human C4 (Complement Component 4) CLIA Kit
SKU:
HUES00144
Target:
Human C4 (Complement Component 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C4. You can calculate the concentration of Human C4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
93-107
88-102
Average (%)
108
100
94
1:4
Range (%)
95-111
97-112
102-114
Average (%)
102
105
108
1:8
Range (%)
89-105
101-118
87-100
Average (%)
97
110
93
1:16
Range (%)
96-108
100-114
97-110
Average (%)
102
107
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
101-113
108
Cell culture media (n=5)
100-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
329.42
570.42
2771.77
338.58
604.86
2670.8
Standard deviation
31.29
67.82
167.97
27.26
71.25
198.17
C V (%)
9.5
11.89
6.06
8.05
11.78
7.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4 in samples. No significant cross-reactivity or interference between Human C4 and analogues was observed.
