The Human C5a Complement Component 5a CLIA Kit is specifically designed for the quantitative detection of C5a levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and accuracy, allowing for precise and reliable results in a variety of research applications.C5a is a key component of the complement system, a crucial part of the immune response that helps to eliminate pathogens and regulate inflammation. Abnormal levels of C5a have been linked to various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases. By measuring C5a levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets.Overall, the Human C5a Complement Component 5a CLIA Kit offers a valuable tool for researchers studying the role of C5a in health and disease. Its high sensitivity, specificity, and reproducibility make it an essential asset for any laboratory conducting research in the field of immunology and inflammation.
Product Name:
Human C5a (Complement Component 5a) CLIA Kit
SKU:
HUES00164
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
