The Human C5a Complement Component 5a ELISA Kit is specifically designed for the precise measurement of C5a levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.C5a is a vital component of the complement system and plays a crucial role in inflammation and immune response. Dysregulation of C5a has been implicated in various inflammatory disorders, autoimmune diseases, and sepsis, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions. With its advanced technology and validated performance, the Human C5a Complement Component 5a ELISA Kit is an essential tool for scientists and researchers looking to investigate the role of C5a in health and disease. Obtain reliable data and insights with this innovative ELISA kit from Assay Genie.
Product Name:
Human C5a (Complement Component 5a) ELISA Kit
SKU:
HUES01457
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
