Description
Human C5AR1 Recombinant Protein (N-GST&C-His)
Human C5AR1 Recombinant Protein (N-GST&C-His) is a recombinant protein expressed in E. coli, spanning Arg175-Arg206, with an N-terminal GST tag and a C-terminal His tag. Predicted molecular weight: 32.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. C5a anaphylatoxin chemotactic receptor 1 is a ~39 kDa protein. Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. For research use only.
Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. C5AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events.
| Product Name: | Human C5AR1 Recombinant Protein (N-GST&C-His) |
| SKU: | GXRP09078 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD88; C5AR1; C5AR; C5R1 |
| Tag: | N-GST&C-His |
| Expression Host: | E. coli |
| Protein Length: | Arg175-Arg206 |
| Calculated MW: | 32.03 kDa |
| UniProt: | P21730 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
