Human CA15-3 PharmaGenie ELISA Kit (SBRS0337)

SKU:
SBRS0337
Product type:
ELISA
Reactivity:
Human
Application:
ELISA
ELISA Type:
Sandwich
€597

Description

Human CA15-3 PharmaGenie ELISA - Kit Data

Product SKU:SBRS0337
Size:96T
Application:This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Uniprot:P15941
Gene ID:4582
Gene Names:MUC1 / PUM
Synonyms:Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha) Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]
Target Species:Human
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:4 mU/ml - 1000 mU/ml
Sensitivity:4 mU/ml
Recommended Dilution:30 - 200 fold
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Stop Solution
4.Assay Diluent(s)
5.Lyophilized Standard
6.Biotinylated Detection Antibody
7.Streptavidin-Conjugated HRP
8.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human CA15-3 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 ul of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 ul of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.
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