Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit
The Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit is specially designed for the accurate measurement of CHST15 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it an invaluable tool for researchers in various fields.CHST15 is an enzyme involved in the sulfation of carbohydrates, playing a crucial role in various biological processes such as cell adhesion, angiogenesis, and inflammation. Dysregulation of CHST15 has been implicated in diseases like cancer, inflammatory disorders, and cardiovascular diseases, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.
With the Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit, researchers can accurately quantify CHST15 levels in biological samples, allowing for in-depth studies on its function and potential implications in various diseases. This kit offers a reliable and efficient method for detecting CHST15, making it an essential tool for advancing research in the field of carbohydrate sulfotransferase biology.
Product Name:
Human Carbohydrate Sulfotransferase 15 (CHST15) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
