Human Carbohydrate Sulfotransferase 9 (CHST9) ELISA Kit
The Human Carbohydrate Sulfotransferase 9 (CHST9) ELISA Kit is a powerful tool for the detection of CHST9 levels in human samples. This kit provides high sensitivity and specificity, delivering accurate and reliable results for various research applications.Carbohydrate sulfotransferase 9 is an enzyme that plays a key role in the sulfation of carbohydrates, which is important for various biological processes including cell adhesion, migration, and signaling. Dysregulation of CHST9 has been linked to a variety of diseases, making it a valuable biomarker for studying conditions such as inflammation, cancer, and autoimmune disorders.
With the Human CHST9 ELISA Kit, researchers can easily quantify CHST9 levels in serum, plasma, and cell culture supernatants, providing valuable insights into the role of this enzyme in health and disease. This kit is a valuable asset for any lab studying carbohydrate sulfotransferases and their impact on human biology.
Product Name:
Human Carbohydrate Sulfotransferase 9 (CHST9) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
