Human Cartilage Associated Protein (CRTAP) ELISA Kit
The Human CRTAP (Cartilage-Associated Protein) ELISA Kit is specifically designed for the precise measurement of CRTAP levels in human serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and specificity, guaranteeing accurate and dependable results for various research endeavors.CRTAP is an essential protein that plays a vital role in the development and maintenance of cartilage. Its involvement in bone formation and collagen synthesis makes it a key player in skeletal health and various bone-related disorders.
By accurately measuring CRTAP levels, researchers can gain valuable insights into skeletal diseases, bone metabolism, and potential therapeutic interventions.Overall, the Human CRTAP ELISA Kit is a valuable tool for researchers studying cartilage-associated proteins and their implications in skeletal health. Its reliability and precision make it an excellent choice for a wide range of research applications in the field of skeletal biology and related disciplines.
Product Name:
Human Cartilage Associated Protein (CRTAP) ELISA Kit
Product Code:
HUDL00688
Size:
96 Assays
Target:
CRTAP
Synonyms:
CASP, LEPREL3, Leprecan-Like 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
