Description
Human CDC27 Recombinant Protein (N-His)
Human CDC27 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn17-Ser231, with an N-terminal His tag. Predicted molecular weight: 26.5 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cell division cycle protein 27 homolog (CDC27) is a ~91 kDa protein. Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. For research use only.
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.
| Product Name: | Human CDC27 Recombinant Protein (N-His) |
| SKU: | GXRP01395 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ANAPC3, Cell division cycle protein 27 homolog, CDC27, APC3, Anaphase-promoting complex subunit 3, CDC27Hs, D0S1430E, CDC27 homolog, D17S978E, H-NUC |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn17-Ser231 |
| Calculated MW: | 26.5 kDa |
| UniProt: | P30260 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
