Description
Human CDK4 Recombinant Protein (N-His)
Human CDK4 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Glu303, with an N-terminal His tag. Predicted molecular weight: 35.88 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cyclin-dependent kinase 4 (CDK4) is a ~33 kDa protein. Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. For research use only.
Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity.
| Product Name: | Human CDK4 Recombinant Protein (N-His) |
| SKU: | GXRP00891 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Cell division protein kinase 4, PSK-J3, Cyclin-dependent kinase 4, CDK4 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Glu303 |
| Calculated MW: | 35.88 kDa |
| UniProt: | P11802 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
