The Human CDK6 (Cyclin-Dependent Kinase 6) CLIA Kit is a powerful tool for accurately quantifying CDK6 levels in human samples including serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.CDK6 is a key enzyme involved in cell cycle regulation and has been linked to various diseases including cancer, making it a valuable biomarker for studying disease mechanisms and potential therapeutic targets.
With the Human CDK6 CLIA Kit, researchers can confidently measure CDK6 levels and advance their understanding of disease pathways.Overall, the Human CDK6 CLIA Kit is an essential tool for researchers looking to investigate the role of CDK6 in disease progression and therapeutic development. Get accurate and reliable results with this advanced kit from Assay Genie.
Product Name:
Human CDK6 (Cyclin Dependent Kinase 6) CLIA Kit
SKU:
HUES00228
Target:
Human CDK6 (Cyclin Dependent Kinase 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CDK6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CDK6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDK6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CDK6. You can calculate the concentration of Human CDK6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
99-113
87-102
Average (%)
106
104
94
1:4
Range (%)
103-116
98-110
95-110
Average (%)
110
104
101
1:8
Range (%)
96-112
99-111
96-109
Average (%)
102
105
102
1:16
Range (%)
100-115
90-104
93-106
Average (%)
108
97
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
108
EDTA plasma (n=5)
101-115
107
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.94
240.96
754.58
110.83
264.01
796.92
Standard deviation
12.25
27.88
73.27
10.57
21.36
85.03
C V (%)
11.56
11.57
9.71
9.54
8.09
10.67
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CDK6 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CDK6 in samples. No significant cross-reactivity or interference between Human CDK6 and analogues was observed.
