Description
Human CDKN2A Recombinant Protein (N-GST & C-His)
Human CDKN2A Recombinant Protein (N-GST & C-His) is a recombinant protein expressed in E. coli, spanning Val2-Arg54, with an N-terminal GST tag and a C-terminal His tag. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Tumor suppressor ARF (CDKN2A/p16INK4a) is a ~13 kDa protein. Capable of inducing cell cycle arrest in G1 and G2 phases. For research use only.
Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes.
| Product Name: | Human CDKN2A Recombinant Protein (N-GST & C-His) |
| SKU: | GXRP04085 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ARF, MLM, p14ARF, Cyclin-dependent kinase inhibitor 2A, CDKN2, CDKN2A, Alternative reading frame, Tumor suppressor ARF |
| Tag: | N-GST & C-His |
| Expression Host: | E. coli |
| Protein Length: | Val2-Arg54 |
| UniProt: | Q8N726 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
