The Human CDSN (Corneodesmosin) CLIA Kit is a specialized assay designed for the precise measurement of CDSN levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.CDSN is a key protein involved in maintaining the barrier function of the skin and plays a crucial role in regulating the desquamation process.
Abnormalities in CDSN expression have been linked to various skin disorders including psoriasis and atopic dermatitis, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human CDSN CLIA Kit is an indispensable tool for researchers seeking to unravel the complex mechanisms underlying skin health and disease, providing valuable insights for the development of novel treatment strategies.
Product Name:
Human CDSN (Corneodesmosin) CLIA Kit
SKU:
HUES00852
Target:
Human CDSN (Corneodesmosin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CDSN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CDSN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDSN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CDSN. You can calculate the concentration of Human CDSN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
90-101
92-104
Average (%)
94
95
97
1:4
Range (%)
94-105
97-115
90-104
Average (%)
99
105
95
1:8
Range (%)
89-99
87-104
87-101
Average (%)
94
95
93
1:16
Range (%)
85-99
98-112
102-117
Average (%)
90
105
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
84-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.52
224.11
744.86
97.36
232.05
739.82
Standard deviation
7.96
25.62
63.69
10.02
22.32
50.83
C V (%)
8.6
11.43
8.55
10.29
9.62
6.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CDSN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CDSN in samples. No significant cross-reactivity or interference between Human CDSN and analogues was observed.
