Description
Human CGAS Recombinant Protein (N-His)
Human CGAS Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser201-Phe522, with an N-terminal His tag. Predicted molecular weight: 40.32 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cyclic GMP-AMP synthase (CGAS) is a ~58 kDa protein. Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (2',3'-cGAMP) from ATP and GTP and plays a key role in innate immunity. For research use only.
Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Acts as a key DNA sensor: directly binds double-stranded DNA (dsDNA), inducing the formation of liquid-like droplets in which CGAS is activated, leading to synthesis of 2',3'-cGAMP, a second messenger that binds to and activates STING1, thereby triggering type-I interferon production. Preferentially recognizes and binds curved long dsDNAs of a minimal length of 40 bp. Acts as a key foreign DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses.
| Product Name: | Human CGAS Recombinant Protein (N-His) |
| SKU: | GXRP06363 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | h-cGAS, MB21D1, 2'3'-cGAMP synthase, cGAS, cGAMP synthase, Mab-21 domain-containing protein 1, Cyclic GMP-AMP synthase, CGAS, C6orf150 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser201-Phe522 |
| Calculated MW: | 40.32 kDa |
| UniProt: | Q8N884 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
