Description
Human CHFR Recombinant Protein (N-His)
Human CHFR Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln14-Lys126, with an N-terminal His tag. Predicted molecular weight: 15.16 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase CHFR is a ~73 kDa protein. E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. For research use only.
Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation.
| Product Name: | Human CHFR Recombinant Protein (N-His) |
| SKU: | GXRP06381 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | E3 ubiquitin-protein ligase CHFR, RNF196, Checkpoint with forkhead and RING finger domains protein, CHFR, RING-type E3 ubiquitin transferase CHFR, RING finger protein 196 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln14-Lys126 |
| Calculated MW: | 15.16 kDa |
| UniProt: | Q96EP1 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
