Description
Human Coagulation Factor X ELISA Kit (SBRS6181)
The Human Coagulation Factor X ELISA Kit is a sandwich enzyme-linked immunosorbent assay for the quantitative measurement of human coagulation factor X in serum and plasma. It uses colorimetric detection at 450 nm to deliver reliable and reproducible quantification of human coagulation factor X for research applications.
The 96-well plate is coated with a capture antibody specific for human coagulation factor X. Factor X present in standards and samples is captured by the immobilized antibody and detected with a biotinylated detection antibody, followed by HRP-conjugated streptavidin and TMB substrate. The Stop Solution changes the color from blue to yellow, and the intensity, read at 450 nm, is proportional to the amount of factor X bound.
| Product SKU: | SBRS6181 |
| Size: | 96T |
| Species: | Human |
| Sample Types: | Plasma, Serum |
| Design Principle: | Sandwich |
| Method of Detection: | Colorimetric |
| Assay Type: | Quantitative |
| Detection Wavelength: | 450 nm |
| UniProt: | P00742 |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see the technical manual. |
| Component | Quantity |
|---|---|
| Human Factor X Microplate (96 wells, 12 x 8) | 1 plate |
| Human Factor X Standard Protein | 2 vials |
| Human Factor X Detection Antibody | 2 vials |
| Wash Buffer (20X) | 25 ml |
| HRP-Streptavidin | 200 µl |
| TMB One-Step Substrate Reagent | 12 ml |
| Stop Solution | 8 ml |
| Assay Diluent(s) | Supplied |
- Prepare all reagents, samples and standards as instructed. Bring all reagents and samples to room temperature before use.
- Add 100 µl of each standard or sample to the appropriate wells.
- Incubate 2.5 hours at room temperature with gentle shaking.
- Wash 4 times with Wash Buffer (1X).
- Add 100 µl of prepared Detection Antibody to each well.
- Incubate 1 hour at room temperature with gentle shaking.
- Wash 4 times with Wash Buffer (1X).
- Add 100 µl of prepared HRP-Streptavidin solution to each well.
- Incubate 45 minutes at room temperature with gentle shaking.
- Wash 4 times with Wash Buffer (1X).
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature in the dark with gentle shaking.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
