The Human COL17 (Collagen Type XVII) CLIA Kit is a cutting-edge assay designed for the precise measurement of COL17 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.COL17, also known as BP180, is a crucial component of the dermal-epidermal junction in the skin, playing a key role in maintaining skin integrity and function. Dysregulation of COL17 has been implicated in various dermatological conditions, including autoimmune disorders and skin aging, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its user-friendly protocol and high-performance capabilities, the Human COL17 CLIA Kit is a valuable tool for researchers seeking to explore the role of COL17 in skin biology and disease pathology. Order yours today and unlock new insights into the intricate mechanisms governing skin health and function.
Product Name:
Human COL17 (Collagen Type XVII) CLIA Kit
SKU:
HUES00495
Target:
Human COL17 (Collagen Type XVII)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL17. You can calculate the concentration of Human COL17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
95-108
86-98
Average (%)
108
102
92
1:4
Range (%)
97-113
88-99
89-102
Average (%)
104
93
94
1:8
Range (%)
89-102
88-101
101-115
Average (%)
95
93
108
1:16
Range (%)
93-107
86-99
94-106
Average (%)
99
92
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
96-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.01
4.03
17.44
2.1
4.14
15.96
Standard deviation
0.22
0.29
1.35
0.26
0.3
1.21
C V (%)
10.95
7.2
7.74
12.38
7.25
7.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed.
