The Human Type IV Collagen (COL4) CLIA Kit is specifically designed for the quantitative determination of Type IV Collagen in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and reliable results for a variety of research purposes.Type IV Collagen is a key component of the basement membrane, essential for tissue structure and function. Abnormal levels of Type IV Collagen have been linked to various diseases including kidney diseases, certain cancers, and autoimmune disorders. Therefore, the detection and measurement of Type IV Collagen levels can provide valuable insights into the progression and treatment of these conditions.With its advanced technology and user-friendly format, the Human Type IV Collagen CLIA Kit from Assay Genie is the perfect tool for researchers seeking to investigate the role of Type IV Collagen in health and disease. Order yours today and take your research to the next level.
Product Name:
Human COL4 (Collagen Type IV) CLIA Kit
SKU:
HUES00152
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
