The Mouse COL4 (Collagen Type IV) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of collagen type IV levels in mouse samples. This kit is ideal for researchers studying extracellular matrix remodeling, basement membrane integrity, and various cellular processes in mice.Collagen type IV is a major component of basement membranes and plays a critical role in maintaining tissue structure and function. Abnormal collagen type IV levels have been associated with various diseases, including kidney disorders, vascular diseases, and muscular dystrophies.
Accurate measurement of collagen type IV levels in mouse samples can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and reliability, the Mouse COL4 CLIA Kit is a valuable tool for researchers investigating the role of collagen type IV in health and disease in mouse models.
Product Name:
Mouse COL4 (Collagen Type IV) CLIA Kit
SKU:
MOES00189
Target:
Mouse COL4 (Collagen Type IV)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL4. You can calculate the concentration of Mouse COL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
89-101
95-109
Average (%)
105
94
101
1:4
Range (%)
91-108
92-109
88-101
Average (%)
98
100
93
1:8
Range (%)
98-110
87-101
96-110
Average (%)
103
92
103
1:16
Range (%)
86-101
98-112
102-118
Average (%)
92
104
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
99
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
1.68
7.01
0.98
1.77
7.17
Standard deviation
0.11
0.15
0.63
0.1
0.17
0.58
C V (%)
11.46
8.93
8.99
10.2
9.6
8.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse COL4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL4 in samples. No significant cross-reactivity or interference between Mouse COL4 and analogues was observed.
