The Human Col4 (Collagen Type IV) ELISA Kit is specifically designed for the precise measurement of collagen type IV levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Collagen type IV is a key component of the basement membrane, crucial for maintaining tissue structure and integrity. Abnormal levels of collagen type IV have been linked to various diseases such as kidney diseases, diabetes, and autoimmune disorders, highlighting its importance as a potential biomarker for disease progression and therapeutic development. This ELISA kit is a valuable tool for researchers studying collagen type IV biology, pathophysiology, and potential therapeutic interventions. With its reliable performance and ease of use, this kit is an essential addition to any lab looking to further explore the role of collagen type IV in human health and disease.
Product Name:
Human COL4 (Collagen Type IV) ELISA Kit
SKU:
HUES01445
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
