The Human COL6 (Collagen Type VI) CLIA Kit is a highly precise assay designed for the quantitative measurement of Collagen Type VI levels in human biological samples. This kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results across a variety of research applications.Collagen Type VI is a key component of the extracellular matrix and is involved in various physiological processes such as tissue repair, cell adhesion, and cell differentiation.
Dysregulation of Collagen Type VI has been linked to several diseases, including muscular dystrophy, arthritis, and fibrosis, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.Overall, the Human COL6 CLIA Kit provides researchers with a reliable tool for investigating the role of Collagen Type VI in health and disease, paving the way for new insights into pathophysiological mechanisms and potential treatment strategies.
Product Name:
Human COL6 (Collagen Type VI) CLIA Kit
SKU:
HUES00485
Target:
Human COL6 (Collagen Type VI)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL6. You can calculate the concentration of Human COL6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
102-121
96-111
Average (%)
101
110
102
1:4
Range (%)
103-117
91-102
100-117
Average (%)
109
96
108
1:8
Range (%)
99-115
96-112
90-103
Average (%)
107
102
98
1:16
Range (%)
95-111
84-97
99-114
Average (%)
101
91
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
95-110
103
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.13
13.66
45.29
5.18
12.76
46.98
Standard deviation
0.64
1.59
4.32
0.53
1.17
3.71
C V (%)
12.48
11.64
9.54
10.23
9.17
7.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL6 in samples. No significant cross-reactivity or interference between Human COL6 and analogues was observed.
