The Human COL7 (Collagen Type VII) CLIA Kit is specifically designed for the quantitative measurement of Collagen Type VII levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, ensuring reliable and reproducible results for a variety of research applications.Collagen Type VII is a key component of the skin's anchoring fibrils, playing a vital role in maintaining the structural integrity of the skin and other connective tissues. Abnormalities in Collagen Type VII have been associated with various skin disorders, including dystrophic epidermolysis bullosa, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
Overall, the Human COL7 (Collagen Type VII) CLIA Kit provides researchers with a powerful tool for studying the role of Collagen Type VII in skin health and disease, offering valuable insights into potential therapeutic approaches for a range of dermatological conditions.
Product Name:
Human COL7 (Collagen Type VII) CLIA Kit
SKU:
HUES00491
Target:
Human COL7 (Collagen Type VII)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL7. You can calculate the concentration of Human COL7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
87-99
96-109
Average (%)
107
94
102
1:4
Range (%)
84-97
95-107
95-110
Average (%)
90
101
102
1:8
Range (%)
94-110
93-107
96-108
Average (%)
100
99
101
1:16
Range (%)
99-115
88-102
91-105
Average (%)
106
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
98-114
105
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.67
298.14
708.17
105.25
327.56
674.86
Standard deviation
10.81
21.14
68.48
12.86
29.68
51.36
C V (%)
10.85
7.09
9.67
12.22
9.06
7.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL7 in samples. No significant cross-reactivity or interference between Human COL7 and analogues was observed.
