The Human Collagenase I CLIA Kit is specifically designed for the quantitative measurement of human collagenase I levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Collagenase I is an important enzyme involved in the breakdown of collagen, a key component of connective tissues in the body. Dysregulation of collagenase I activity has been linked to various diseases such as arthritis, fibrosis, and cancer, highlighting the significance of this enzyme as a potential biomarker for studying these conditions.
With its advanced technology and easy-to-use format, the Human Collagenase I CLIA Kit is an essential tool for researchers looking to investigate the role of collagenase I in health and disease, ultimately leading to a better understanding of pathophysiological mechanisms and potential therapeutic targets.
Product Name:
Human Collagenase I CLIA Kit
SKU:
HUES00496
Target:
Human Collagenase I
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Collagenase I. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Collagenase I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Collagenase I, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Collagenase I. You can calculate the concentration of Human Collagenase I in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
95-111
101-115
Average (%)
100
103
107
1:4
Range (%)
100-117
100-118
89-99
Average (%)
108
108
94
1:8
Range (%)
100-116
95-111
87-99
Average (%)
106
103
92
1:16
Range (%)
102-114
91-105
86-101
Average (%)
108
96
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.36
184.94
860.08
83.72
183.61
883.1
Standard deviation
8.67
16.39
83.17
9.64
17.06
59.87
C V (%)
9.49
8.86
9.67
11.51
9.29
6.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Collagenase I concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Collagenase I in samples. No significant cross-reactivity or interference between Human Collagenase I and analogues was observed.
