Description
Human CTCF Recombinant Protein (N-His)
Human CTCF Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Ile154, with an N-terminal His tag. Predicted molecular weight: 19.09 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Transcriptional repressor CTCF is a ~82 kDa protein. Chromatin binding factor that binds to DNA sequence specific sites and regulates the 3D structure of chromatin. For research use only.
Binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures, such as the nuclear lamina. Defines the boundaries between active and heterochromatic DNA via binding to chromatin insulators, thereby preventing interaction between promoter and nearby enhancers and silencers. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2.
| Product Name: | Human CTCF Recombinant Protein (N-His) |
| SKU: | GXRP01694 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Transcriptional repressor CTCF, CTCF, CCCTC-binding factor, 11-zinc finger protein, CTCFL paralog |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Ile154 |
| Calculated MW: | 19.09 kDa |
| UniProt: | P49711 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
