Description
Human CYP1A2 Recombinant Protein (N-His)
Human CYP1A2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala2-Ser231, with an N-terminal His tag. Predicted molecular weight: 27.45 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cytochrome P450 1A2 (CYP1A2) is a ~58 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. For research use only.
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis.
| Product Name: | Human CYP1A2 Recombinant Protein (N-His) |
| SKU: | GXRP00595 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Hydroperoxy icosatetraenoate dehydratase, Cytochrome P450-P3, Cytochrome P450 4, CYP1A2, Cholesterol 25-hydroxylase, CYPIA2, Cytochrome P(3)450, Cytochrome P450 1A2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala2-Ser231 |
| Calculated MW: | 27.45 kDa |
| UniProt: | P05177 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
