Description
Human CYP3A4 Recombinant Protein (N-His)
Human CYP3A4 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly26-Ala503, with an N-terminal His tag. Predicted molecular weight: 56.79 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cytochrome P450 3A4 (CYP3A4) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. For research use only.
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position. Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone.
| Product Name: | Human CYP3A4 Recombinant Protein (N-His) |
| SKU: | GXRP03369 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CYPIIIA3, Cytochrome P450 3A4, 1, 4-cineole 2-exo-monooxygenase, CYP3A4, Cytochrome P450 3A3, Cytochrome P450 NF-25, Cytochrome P450-PCN1, CYPIIIA4, 1, 8-cineole 2-exo-monooxygenase, Albendazole monooxygenase (sulfoxide-forming), Nifedipine oxidase, Quinine 3-monooxygenase, CYP3A3, Cholesterol 25-hydroxylase, Cytochrome P450 HLp, Albendazole sulfoxidase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly26-Ala503 |
| Calculated MW: | 56.79 kDa |
| UniProt: | P08684 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
