Description
Human DEFB4A Recombinant Protein (N-GST)
Human DEFB4A Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Gly24-Pro64, with an N-terminal GST tag. Predicted molecular weight: 31.16 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Defensin beta 4A (DEFB4A) is a ~7 kDa protein. Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria. For research use only.
Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM. Also exhibits antimicrobial activity against the yeast C.albicans. Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death. Acts as a ligand for C-C chemokine receptor CCR6.
| Product Name: | Human DEFB4A Recombinant Protein (N-GST) |
| SKU: | GXRP00114 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | SAP1, Beta-defensin 4A, DEFB4, hBD-2, BD-2, Skin-antimicrobial peptide 1, Beta-defensin 2, Defensin, beta 2, DEFB2, DEFB4A, DEFB102 |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Gly24-Pro64 |
| Calculated MW: | 31.16 kDa |
| UniProt: | O15263 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
