Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit
The Human Dual Specificity Phosphatase-1 (DUSP1) ELISA Kit is a highly reliable and accurate tool for the detection of DUSP1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.DUSP1 is an important enzyme involved in regulating cell growth, differentiation, and immune response. It plays a critical role in various biological processes and has been linked to numerous diseases, including cancer, inflammatory disorders, and autoimmune conditions.
By accurately measuring DUSP1 levels, researchers can better understand its function and explore potential therapeutic interventions for related conditions.With its advanced technology and proven performance, the Human DUSP1 ELISA Kit is an invaluable tool for researchers seeking to investigate the role of DUSP1 in health and disease. Order your kit today and unlock new insights into this essential protein's biological functions and therapeutic potential.
Product Name:
Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit
Product Code:
HUDL00888
Size:
96 Assays
Target:
DUSP1
Synonyms:
CL100, HVH1, MKP1, PTPN10, Mitogen-activated protein kinase phosphatase 1, Protein-tyrosine phosphatase CL100, Dual specificity protein phosphatase hVH1
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
