The Human DUSP5 VH3 ELISA Kit is a highly reliable and sensitive assay designed for the precise quantitative measurement of DUSP5 levels in human serum, plasma, and cell culture supernatants. DUSP5, also known as Dual-specificity phosphatase 5, is a key enzyme that plays a critical role in regulating cell growth and proliferation. This ELISA kit offers high specificity and accuracy, ensuring reproducible results for a wide range of research applications. By detecting DUSP5 levels, researchers can gain valuable insights into various biological processes, including cell signaling pathways and gene expression regulation.
DUSP5 is implicated in a variety of diseases, including cancer, inflammatory disorders, and cardiovascular diseases. By studying the expression levels of DUSP5, researchers can identify potential therapeutic targets and develop innovative treatment strategies. Overall, the Human DUSP5 VH3 ELISA Kit is an essential tool for researchers studying the role of DUSP5 in health and disease, offering reliable and precise measurements for impactful research outcomes.
Product Name:
Human DUSP5/VH3 ELISA Kit
SKU:
HUFI00670
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
93-101%
82-100%
81-100%
EDTA Plasma (n = 5)
86-103%
84-91%
82-90%
Heparin Plasma (n = 5)
90-104%
87-100%
80-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
89-98
94
EDTA Plasma (n = 5)
86-102
92
Heparin Plasma (n = 5)
88-105
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
