Human EDN (EosinophILDerived Neurotoxin) ELISA Kit
The Human EDN (Eosinophil-Derived Neurotoxin) ELISA Kit is a powerful tool for the quantitative measurement of EDN levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results, making it ideal for a wide range of research applications.EDN is a protein released by eosinophils, a type of white blood cell, and has been implicated in various inflammatory and allergic conditions.
Its role in neurotoxicity and immune responses makes it a valuable biomarker for studying these processes and potentially developing new therapies.Overall, the Human EDN ELISA Kit offers researchers a precise and efficient method for studying the role of EDN in health and disease, providing valuable insights into inflammatory and immune-related conditions.
Product Name:
Human EDN (EosinophILDerived Neurotoxin) ELISA Kit
SKU:
HUES03216
Target:
Human EDN (EosinophILDerived Neurotoxin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EDN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EDN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EDN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EDN. You can calculate the concentration of Human EDN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
86-100
86-97
Average (%)
105
93
92
1:4
Range (%)
91-104
82-95
86-99
Average (%)
98
89
92
1:8
Range (%)
87-101
86-98
85-96
Average (%)
93
91
91
1:16
Range (%)
89-101
85-96
87-101
Average (%)
95
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
83-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.91
5.96
17.11
1.73
5.46
18.77
Standard deviation
0.12
0.25
0.63
0.09
0.26
0.68
C V (%)
6.28
4.19
3.68
5.2
4.76
3.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human EDN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EDN in samples. No significant cross-reactivity or interference between Human EDN and analogues was observed.
