Description
Human EEFSEC Recombinant Protein (N-His)
Human EEFSEC Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Ala305, with an N-terminal His tag. Predicted molecular weight: 34.63 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Selenocysteine-specific elongation factor is a ~65 kDa protein. Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons. For research use only.
Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur.
| Product Name: | Human EEFSEC Recombinant Protein (N-His) |
| SKU: | GXRP11376 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:3.6.5.-, EEFSEC, Elongation factor sec, Eukaryotic elongation factor, selenocysteine-tRNA-specific, SELB, Selenocysteine-specific elongation factor |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Ala305 |
| Calculated MW: | 34.63 kDa |
| UniProt: | P57772 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
