Human Elastin Microfibril Interface Located Protein 2 (EMILIN2) ELISA Kit
The Human EMILIN2 (Elastin Microfibril Interface-Located Protein 2) ELISA Kit is a powerful tool for the precise measurement of EMILIN2 levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.EMILIN2 is a key protein found at the interface of elastin microfibrils, playing a crucial role in maintaining the structural integrity of extracellular matrix components. Dysregulation of EMILIN2 has been implicated in numerous pathological conditions, including fibrosis, cardiovascular diseases, and connective tissue disorders.
Therefore, detecting and quantifying EMILIN2 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With the Human EMILIN2 ELISA Kit, researchers can gain a deeper understanding of the role of EMILIN2 in health and disease, paving the way for new diagnostic and therapeutic strategies in various medical fields.
Product Name:
Human Elastin Microfibril Interface Located Protein 2 (EMILIN2) ELISA Kit
Product Code:
HUDL00938
Size:
96 Assays
Target:
EMILIN2
Synonyms:
FOAP-10, Elastin Microfibril Interfacer 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
