Human eNOS/Nitric oxide synthase, endothelial ELISA Kit
The Human eNOS (Nitric Oxide Synthase) Endothelial ELISA Kit is specifically designed for the accurate measurement of eNOS levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, providing valuable insights into endothelial function and nitric oxide production in various research applications. Endothelial nitric oxide synthase (eNOS) is a critical enzyme responsible for the production of nitric oxide, a key signaling molecule involved in the regulation of blood pressure, vascular tone, and overall cardiovascular health.
Dysregulation of eNOS activity has been implicated in numerous cardiovascular diseases, making it a crucial biomarker for studying endothelial dysfunction and developing potential therapeutic interventions. By using the Human eNOS Endothelial ELISA Kit, researchers can accurately assess eNOS levels in biological samples, leading to a better understanding of the role of nitric oxide in various physiological and pathological processes. This kit is a valuable tool for advancing cardiovascular research and exploring novel treatment strategies for cardiovascular disorders.
Product Name:
Human eNOS/Nitric oxide synthase, endothelial ELISA Kit
SKU:
HUFI00401
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
37.5 pg/mL
Range:
62.5-4000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-104%
89-103%
86-99%
EDTA Plasma (n = 5)
82-95%
89-100%
82-91%
Heparin Plasma (n = 5)
86-96%
81-99%
84-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-103
95
EDTA Plasma (n = 5)
86-105
99
Heparin Plasma (n = 5)
90-104
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
