Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
The Human NOS3 (eNOS) ELISA Kit is specifically designed for the precise quantification of nitric oxide synthase 3 (NOS3) levels in human endothelial cells, serum, and plasma samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research experiments.NOS3, also known as endothelial nitric oxide synthase (eNOS), is a critical enzyme involved in the production of nitric oxide, a key signaling molecule in the cardiovascular system. Dysregulation of NOS3 activity has been linked to various cardiovascular diseases, such as hypertension and atherosclerosis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.By using the Human NOS3 ELISA Kit, researchers can gain valuable insights into the role of NOS3 in endothelial function and cardiovascular health, paving the way for innovative research and treatment strategies in the field of cardiovascular medicine.
Product Name:
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
SKU:
HUES01899
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
