Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
The Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit is a specialized assay designed to quantitatively detect the levels of endothelial nitric oxide synthase (eNOS), also known as NOS3, in various rat biological samples. Endothelial nitric oxide synthase is a critical enzyme that plays a pivotal role in endothelial function and vascular health by catalyzing the production of nitric oxide, a key signaling molecule involved in vascular tone regulation, blood pressure control, and cardiovascular homeostasis. The accurate quantification of eNOS levels using this ELISA kit can provide valuable insights into endothelial function, vascular integrity, and the pathophysiology of cardiovascular diseases. eNOS dysfunction or dysregulation is associated with various cardiovascular disorders, such as hypertension, atherosclerosis, and coronary artery disease, making it an important target for research and therapeutic interventions. The Rat NOS3/eNOS ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and is user-friendly, making it an excellent choice for researchers investigating endothelial function, vascular biology, and cardiovascular health.
Product Name:
Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
SKU:
AEES00393
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
