Human Epstein Barr Virus Induced Protein 3 (EBI3) ELISA Kit
The Human Epstein-Barr Virus-Induced Protein 3 (EBI3) ELISA Kit is specifically designed to accurately measure EBI3 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures dependable and repeatable results, making it perfect for a variety of research applications.EBI3 is a critical protein that is induced by the Epstein-Barr virus, playing a key role in immune response regulation and inflammation. Studies have shown that EBI3 is involved in various diseases, including autoimmune conditions and certain types of cancer.
By measuring EBI3 levels, researchers can gain valuable insights into the mechanisms of these diseases and potentially develop new therapeutic approaches.Overall, the Human Epstein-Barr Virus-Induced Protein 3 (EBI3) ELISA Kit is a valuable tool for researchers looking to study the role of EBI3 in disease development and progression. Its accuracy and reliability make it an essential addition to any laboratory conducting research in this field.
Product Name:
Human Epstein Barr Virus Induced Protein 3 (EBI3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
