Mouse Epstein Barr Virus Induced Protein 3 (EBI3) ELISA Kit
The Mouse Epstein-Barr Virus Induced Protein 3 (EBI3) ELISA Kit is a specialized assay designed for the accurate quantification of EBI3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.EBI3 is a key protein that is induced by the Epstein-Barr virus and plays a critical role in immune regulation and inflammation. It is involved in the modulation of T-cell responses and the regulation of cytokine production, making it a valuable biomarker for studies related to immune system function, viral infections, and inflammatory diseases.
By utilizing the Mouse Epstein-Barr Virus Induced Protein 3 (EBI3) ELISA Kit, researchers can accurately quantify EBI3 levels in mouse samples, leading to a better understanding of its role in various physiological and pathological processes. This kit is an essential tool for investigating the mechanisms underlying immune responses and developing potential therapeutic interventions.
Product Name:
Mouse Epstein Barr Virus Induced Protein 3 (EBI3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
