SynonymsHS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,MAPK3,ERT2,Mitogen-Activated Protein Kinase 3,Insulin-stimulated MAP2 kinase,Microtubule-associated protein 2 kinase
ReactivityHuman
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
HUDL00973
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27361
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,MAPK3,ERT2,Mitogen-Activated Protein Kinase 3,Insulin-stimulated MAP2 kinase,Microtubule-associated protein 2 kinase
Human Extracellular Signal Regulated Kinase 1 (ERK1) ELISA Kit
The Human Extracellular Signal-Regulated Kinase 1 (ERK1) ELISA Kit is a powerful tool for accurate detection of ERK1 levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ERK1 is a key protein in the MAPK signaling pathway, regulating cellular processes such as cell growth, differentiation, and survival. Dysregulation of ERK1 has been implicated in various diseases, including cancer, inflammatory disorders, and neurological conditions.
Therefore, measuring ERK1 levels is crucial for understanding disease mechanisms and developing targeted therapies.With the Human ERK1 ELISA Kit, researchers can easily quantify ERK1 levels in samples, providing valuable insights into cellular signaling pathways and potential therapeutic targets. This kit is a valuable asset for scientists studying signal transduction pathways and seeking to uncover new insights into disease mechanisms.
Product Name:
Human Extracellular Signal Regulated Kinase 1 (ERK1) ELISA Kit
Product Code:
HUDL00973
Size:
96 Assays
Target:
ERK1
Synonyms:
HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
