SynonymsHS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,MAPK3,ERT2,Mitogen-Activated Protein Kinase 3,Insulin-stimulated MAP2 kinase,Microtubule-associated protein 2 kinase
ReactivityRat
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
RTDL00339
UPC:
Product Type:
ELISA Kit
Sensitivity:
0.249ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,MAPK3,ERT2,Mitogen-Activated Protein Kinase 3,Insulin-stimulated MAP2 kinase,Microtubule-associated protein 2 kinase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Rat Extracellular Signal Regulated Kinase 1 (ERK1) ELISA Kit
The Rat Extracellular Signal-Regulated Kinase 1 (ERK1) ELISA Kit is a powerful tool for measuring ERK1 levels in rat serum, plasma, and cell culture supernatants with high accuracy and precision. This kit boasts exceptional sensitivity and specificity, ensuring dependable and repeatable results for various research applications.ERK1 is a key signaling molecule involved in cell growth, differentiation, and survival, playing a critical role in cellular response to external stimuli. Dysregulation of ERK1 signaling has been implicated in various diseases, including cancer, inflammatory disorders, and neurodegenerative conditions.
Therefore, detecting and monitoring ERK1 levels can provide valuable insights into disease progression and potential therapeutic targets.With the Rat ERK1 ELISA Kit, researchers can explore the intricate mechanisms of ERK1 signaling in rat models, advancing our understanding of disease pathophysiology and paving the way for innovative treatment strategies. Trust in the reliability and accuracy of this ELISA kit to support your research endeavors and uncover new discoveries in the field of signaling pathways and disease mechanisms.
Product Name:
Rat Extracellular Signal Regulated Kinase 1 (ERK1) ELISA Kit
Product Code:
RTDL00339
Size:
96 Assays
Target:
ERK1
Synonyms:
HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
