Human Fas Apoptotic Inhibitory Molecule 3 (FAIM3) ELISA Kit
The Human FAIM3 (Fas apoptotic inhibitory molecule 3) ELISA Kit is a powerful tool for accurately measuring FAIM3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.FAIM3 is a key protein that plays a critical role in inhibiting cell apoptosis, making it a vital factor in cell survival and immune response regulation. Dysregulation of FAIM3 has been associated with various diseases, including cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for understanding the pathogenesis of these diseases and developing targeted treatments.
The Human FAIM3 ELISA Kit from Assay Genie offers researchers a reliable and efficient way to study FAIM3 expression levels and explore its role in disease progression. With its advanced technology and easy-to-use protocols, this kit is a valuable asset for laboratories focused on unraveling the complexities of FAIM3 signaling pathways and its implications for human health.
Product Name:
Human Fas Apoptotic Inhibitory Molecule 3 (FAIM3) ELISA Kit
Product Code:
HUDL01013
Size:
96 Assays
Target:
FAIM3
Synonyms:
TOSO, FCMR, Regulator of Fas-induced apoptosis Toso
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
