Description
Human FBXO31 Recombinant Protein (N-His)
Human FBXO31 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Pro71-Ile289, with an N-terminal His tag. Predicted molecular weight: 28.31 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. F-box only protein 31 is a ~60 kDa protein. Substrate-recognition component of the SCF(FBXO31) protein ligase complex, which specifically mediates the ubiquitination of proteins amidated at their C-terminus in response to oxidative stress, leading to their degradation by the proteasome. For research use only.
FBXO31 specifically recognizes and binds C-terminal peptides bearing an amide: C-terminal amidation in response to oxidative stress takes place following protein fragmentation. The SCF(FBXO31) also plays a role in G1 arrest following DNA damage by mediating ubiquitination of phosphorylated cyclin-D1 (CCND1), promoting its degradation by the proteasome, resulting in G1 arrest. The SCF(FBXO31) complex is however not a major regulator of CCND1 stability during the G1/S transition. In response to genotoxic stress, the SCF(FBXO31) complex directs ubiquitination and degradation of phosphorylated MDM2, thereby promoting p53/TP53-mediated DNA damage response.
| Product Name: | Human FBXO31 Recombinant Protein (N-His) |
| SKU: | GXRP11587 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | F-box only protein 31, FBX14, FBX31, FBXO31 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Pro71-Ile289 |
| Calculated MW: | 28.31 kDa |
| UniProt: | Q5XUX0 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
