Human FGF21 (Fibroblast Growth Factor 21) CLIA Kit
The Human FGF21 (Fibroblast Growth Factor 21) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of FGF21 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.FGF21 is a key regulator of metabolism and energy homeostasis, playing a critical role in conditions such as obesity, diabetes, and metabolic syndrome. By measuring FGF21 levels, researchers can gain valuable insights into these metabolic disorders and potential therapeutic targets.Overall, the Human FGF21 CLIA Kit is a valuable tool for studying the role of FGF21 in metabolic diseases and developing novel treatment strategies.
Product Name:
Human FGF21 (Fibroblast Growth Factor 21) CLIA Kit
SKU:
HUES00072
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
