Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit
The Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit is a powerful tool for the precise measurement of FGF21 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is carefully designed to provide high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.FGF21 is a key protein involved in metabolic regulation, playing a crucial role in energy homeostasis, glucose metabolism, and lipid metabolism. Dysregulation of FGF21 has been linked to metabolic disorders such as obesity, diabetes, and fatty liver disease, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. With its reliable performance and versatile applications, the Human FGF21 ELISA Kit is an essential tool for researchers investigating the role of FGF21 in metabolic diseases, aging, and related disorders. Unlock new insights into FGF21 biology and disease mechanisms with this advanced ELISA kit from Assay Genie.
Product Name:
Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit
SKU:
HUES01361
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
