Description
Human FOXA1 Recombinant Protein (N-His)
Human FOXA1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met253-Ser472, with an N-terminal His tag. Predicted molecular weight: 24.61 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Hepatocyte nuclear factor 3-alpha (FOXA1) is a ~49 kDa protein. Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. For research use only.
Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes.
| Product Name: | Human FOXA1 Recombinant Protein (N-His) |
| SKU: | GXRP01840 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | HNF-3-alpha, Transcription factor 3A, TCF3A, HNF3A, HNF-3A, FOXA1, TCF-3A, Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met253-Ser472 |
| Calculated MW: | 24.61 kDa |
| UniProt: | P55317 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
