The Human FoxP3 (Forkhead Box Protein P3) ELISA Kit is specifically designed for the accurate quantification of FoxP3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.FoxP3 is a key regulatory protein critical for maintaining immune tolerance and controlling the development of regulatory T cells. Dysregulation of FoxP3 expression has been linked to autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a biomarker for studying these conditions and exploring therapeutic interventions.
With its advanced technology and user-friendly protocols, the Human FoxP3 ELISA Kit from AssayGenie is an invaluable tool for researchers seeking to investigate the role of FoxP3 in health and disease. Order yours today to enhance your research capabilities and advance scientific knowledge in the field of immunology.
Product Name:
Human FOXP3 (Forkhead Box Protein P3) ELISA Kit
SKU:
HUES02197
Target:
Human FOXP3 (Forkhead Box Protein P3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FOXP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FOXP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FOXP3. You can calculate the concentration of Human FOXP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
96-108
90-101
Average (%)
94
101
96
1:4
Range (%)
89-105
81-91
86-102
Average (%)
96
86
94
1:8
Range (%)
94-110
83-99
83-96
Average (%)
100
90
89
1:16
Range (%)
92-106
80-94
86-101
Average (%)
98
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-111
101
EDTA plasma (n=5)
95-109
100
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.68
8.0
1.07
2.58
7.85
Standard deviation
0.05
0.11
0.43
0.06
0.11
0.34
C V (%)
5.1
4.1
5.38
5.61
4.26
4.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FOXP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FOXP3 in samples. No significant cross-reactivity or interference between Human FOXP3 and analogues was observed.
