The Human FoxP3 (Forkhead Box Protein P3) ELISA Kit is specifically designed for the accurate quantification of FoxP3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.FoxP3 is a key regulatory protein critical for maintaining immune tolerance and controlling the development of regulatory T cells. Dysregulation of FoxP3 expression has been linked to autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a biomarker for studying these conditions and exploring therapeutic interventions. With its advanced technology and user-friendly protocols, the Human FoxP3 ELISA Kit from AssayGenie is an invaluable tool for researchers seeking to investigate the role of FoxP3 in health and disease. Order yours today to enhance your research capabilities and advance scientific knowledge in the field of immunology.
Product Name:
Human FOXP3 (Forkhead Box Protein P3) ELISA Kit
SKU:
HUES02197
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
