Human GARS/Glycyl tRNA Synthetase GARS protein ELISA Kit
The Human GARS (Glycyl-tRNA Synthetase) ELISA Kit is a specialized tool designed for the accurate measurement of GARS protein levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this ELISA kit offers reliable and reproducible results, making it ideal for a variety of research applications. GARS, also known as glycyl-tRNA synthetase, is a critical enzyme involved in protein synthesis, linking glycine amino acids to their corresponding tRNA molecules. Dysregulation of GARS has been linked to various disorders such as Charcot-Marie-Tooth disease and distal spinal muscular atrophy, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.
Whether investigating the role of GARS in normal cellular function or exploring its implications in disease pathology, the Human GARS ELISA Kit provides researchers with a valuable tool for precise quantification and analysis of GARS protein levels. Trust in this kit to deliver accurate results and advance your research efforts in the field of protein synthesis and neurodegenerative disorders.
Product Name:
Human GARS/Glycyl tRNA Synthetase GARS protein ELISA Kit
SKU:
HUFI02487
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-103%
85-105%
94-101%
EDTA Plasma (n = 5)
92-100%
83-101%
83-96%
Heparin Plasma (n = 5)
82-96%
85-98%
87-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
91-102
99
EDTA Plasma (n = 5)
90-105
97
Heparin Plasma (n = 5)
85-102
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
