Human GDF11 (Growth Differentiation Factor 11) CLIA Kit
The Human GDF11 (Growth Differentiation Factor 11) CLIA Kit is a cutting-edge diagnostic tool for the precise measurement of GDF11 levels in human samples such as serum, plasma, and cell culture supernatants. This kit stands out for its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.GDF11 is a key protein involved in tissue regeneration and has been linked to numerous age-related diseases and conditions.
By accurately measuring GDF11 levels, researchers can gain valuable insights into the role of this protein in aging, cardiac function, skeletal muscle regeneration, and more. This kit provides a valuable tool for studying the potential therapeutic applications of GDF11 and its role in various physiological processes.
Product Name:
Human GDF11 (Growth Differentiation Factor 11) CLIA Kit
SKU:
HUES01053
Target:
Human GDF11 (Growth Differentiation Factor 11)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GDF11. You can calculate the concentration of Human GDF11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
95-108
94-108
Average (%)
104
101
102
1:4
Range (%)
98-112
94-112
101-118
Average (%)
104
102
110
1:8
Range (%)
101-114
91-105
88-102
Average (%)
107
98
96
1:16
Range (%)
86-100
88-100
90-103
Average (%)
92
95
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
90
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.25
45.85
228.71
25.83
41.89
226.12
Standard deviation
2.7
4.65
23.49
2.24
4.71
23.92
C V (%)
10.29
10.14
10.27
8.67
11.24
10.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GDF11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF11 in samples. No significant cross-reactivity or interference between Human GDF11 and analogues was observed.
