Description
Human GEN1 Recombinant Protein (N-His)
Human GEN1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Pro473, with an N-terminal His tag. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Flap endonuclease GEN homolog 1 is a ~102 kDa protein. Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. For research use only.
Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction.
| Product Name: | Human GEN1 Recombinant Protein (N-His) |
| SKU: | GXRP13166 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:3.1.-.-, Flap endonuclease GEN homolog 1, GEN1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Pro473 |
| UniProt: | Q17RS7 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
